奥科公司测序部通用引物合成

引物名称

引物序列

T7

TAATACGACTCACTATAGGG

SP6

5'ATTTAGGTGACACTATAG3'

M13F

5'TGTAAAACGACGGCCAGT3'

M13R

5'CAGGAAACAGCTATGACC3'

T7（17BASE）

5'ACATCCACTTTGCCTTTCTC3'

T7TER

5'TGCTAGTTATTGCTCAGCGG3'

S.TAG

5'CGAACGCCAGCACATGGACA3'

T3

5'ATTAACCCTCACTAAAGGGA3'

M13F（47）

5'CGCCAGGGTTTTCCCAGTCACGAC3'

M13R（48）

5'AGCGGATAACAATTTCACACAGGA3'

PGEX5’

5'GGGCTGGCAAGCCACGTTTGGTG3'

PGEX3’

5'CCGGGAGCTGCATGTGTCAGAGG3'

PEGFPC5’

5'CATGGTCCTGCTGGAGTTCGTG3'

PEGFPC3’

5'TATGGCTGATTATGATCAGT3'

PEGFPN5’

 5'TGGGAGGTCTATATAAGCAGAG3'

PEGFPN3’

 5'CGTCGCCGTCCAGCTCGACCAG3'

PBV220F

 5'AAGAAGGGCAGCATTCAAAG3'

PBV220R

 5'CTG CGT TCT GAT TTA ATC TG3'

5’AOX

5'GACTGGTTCCAATTGACAAGC3'

3’AOX

5'GGCAAATGGCATTCTGACAT3'

AFACTOR

 5'TAC TAT TGC CAG CAT TGC TGC3'

M13(96)

5'CCCTCATAGTTAGCGTAACG3'

PCMVF-BD

 5'TCTAAAAGCTGCGGAATTGT3'

PCMVR

5'TCCAAACTCATCAATGTATC3'

P17110

5’TACCACTACAATGGATG3’

P12584

5’TTTTCAGTATCTACGAT3’

PAS21F

5' TCATCGGAAGCGAGTAG3'

PAS21R

5' CGTTTTAAAACCTAAGAGTCAC3'

3'AD

5' AGA TGG TGC ACG ATG CAC AG3'

3'BD

5'TAA GAG TCA CTT TAA AAT TTG TAT C3'

PQE30F

5' TGA GCG GAT AAC AAT TTC AC3'

PQE30R

 5' GTT CTG AGG TCA TTA CTG G3'

S1

5’CAA CGT GAA AAA ATT ATT ATT CGC3’

S6

5’GTA AAT GAA TTT TCT GTA TGA GG3’

GLP1

5'  TGT ATC TTA TGG TAC TGT AAC TG3'

GLP2

5' CTT TAT GTT TTT GGC GTC TTC CA3'

RVP3

5' CTA GCA AAA TAG GCT GTC CC3'

RVP4

5'GAC GAT AGT CAT GCC CCG CG3'

PTRC99CF

5’TTG CGC CGA CAT CAT AAC3’

PTRC99CR

5’CTG CGT TCT GAT TTA ATC TG3’

PIRES2EGFPF

5'GTA GGC GTG TAC GGT GGG AG3'

PIRES3EGFPR

5'AAC GCA CAC CGG CCT TAT TC3'

PCDNA3.0F2

5'CTC TGG CTA ACT AGA GAA CC3'

PCDNA3.0R2

 5'GGC AAC TAG AAG GCA CAG TC3'

BAC1

 5'AAC CAT CTC GCA AAT AAA TA 3'

BAC2

 5'ACG CAC AGA ATC TAG CGC TT 3'

PB42ADF

5'CCAGCCTCTTGCTGAGTGGAGATG3'

PB42ADR

5'AAGCCGACAACCTTGATTGGAG3'

TRXF

5'CATATGAGCGATAAAATTATTCAC3'

TRXR

5'GGATCCCTTGTCATCGTCATCACCA3'

pTARGETSEQ

5’ TTA CGC CAA GTT ATT TAG GTG ACA3’

pMALC2XF

5’TGC GTA CTG CGG TGA TCA AC3’

pMALC2XR

5’CTG CAA GGC GAT TAA GTT GG3’

pbdgal4f

5'GCCTCTAACATTGAGACAGC3'

pbdgal4r

5'AAGAGTTACTCAAGAACAAGAA3'

pbi121(35s)

5'GACGCACAATCCCACTATCC3'

PEFF

5' TCA AGC CTC AGA CAG TGG TTC3'

PCEPF

5' AGA GCT CGT TTA GTG AAC CG3'

EBVR

5' GTG GTT TGT CCA AAC TCA TC3'

CMVF

5'CGCAAATGGGCGGTAGGCGTG3'

CMVR

5'GTTCACGGTGCCCTCC3'

PTRCHISAF

5’CAT GGT ATG GCT AGC ATG AC3’

PTRCHISAR

5’CAG GCT GAA AAT CTT CTC TC3’

pDC316 F

 5' -TAA CTT GTT TAT TGC AGC -3'

pDC316 A

5' -TAT GCT TAC CGT AAC TTG -3'

BGH

5'TAG AAG GCA CAG TCG AGG3'

pDC316F

5' acg tgg gta taa gag gcg 3'

pDC316R

5' cga tgc tag acg atc cag 3'

pshuttle-cmv F

 5'-ggT CTA TAT AAg CAg AgC Tg-3'

pshuttle-cmv R

 5'-gTg gTA Tgg CTg ATT ATg ATC Ag-3'

GAL4BD

 5'TCA TCG GAA GAG AGT AG 3'

TADH

 5'GTA AAT TTC TGG CAA GGT AGA C 3'

pDsRed-n5'

GACTTTCCAAAATGTCGTAA

T7-EEV

 5'AAG GCT AGA GTA CTT AAT ACG A 3'

pfastBac-5'

GTC CGA AAC CAT GTC GTA C

pfastBac-3'

CTG ATT ATG ATC CTC TAG TAC TTC

PBAD5'

TAT CGC AAC TCT CTA CTG TT

PBAD3'

5' AGC CAA GCT GGA GAC CGT TT 3'

5'AD

AAT ACC ACT ACA ATG GAT GAT G

N-CMV-30

ATA ACC CCG CCC CGT TG

C-CMV-24

TTA GGA CAA GGC TGG TGG

pBABE_5'

ctt tat cca gcc ctc ac

pBABE_3' 

accctaactgacacacattcc

P5'

TGC GTA CTG CGG TGA TCA AC

P3'

CTG CAA GGC GAT TAA GTT GG

 DuetDOWN1

 5'-GATTATGCGGCCGTGTACAA-3'

PET Upstream

ATG CGT CCG GCG TAG AGG AT

DuetUP2

5'-TTGTACACGGCCGCATAATC-3'

pSR 5' Primer

ggaagccttggcttttg

SV40 PolyA

5'  TAAGATACATTGATGAGTTTGGAC  3'

pCMV5R   

ATTATAgAggACACCTAgTC

pCMV5F   

TTCCAAAATgTCgTAATAAC